Insulin Like Growth Factor-1 Active Protein | mIGF1 active protein

Recombinant Mouse Insulin Like Growth Factor-1

Cat. Num. AAA38121

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor-1; N/A; Recombinant Mouse Insulin Like Growth Factor-1; IGF1 Mouse; Insulin-Like Growth Factor 1 Mouse Recombinant; Somatomedin C; IGF-I; IGFIA; IGF1; mIGF1 active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized with no additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu
• Sequence Length: 153

• Solubility: It is recommended to reconstitute the lyophilized IGF1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF-I as a Reference Standard.
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.
• Preparation and Storage: Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGF-1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mIGF1 active protein

Description: Insulin-Like Growth Factor I mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by proprietary chromatographic techniques.

Introduction: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

Product Categories/Family for mIGF1 active protein

Cytokines and Growth Factors; Growth Factors; Insulin-Like Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 163659894
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104745.1
• NCBI GenBank Nucleotide #: NM_001111275.1
• UniProt Accession #: P05017; P05018; Q6LDP4; Q8C4U6
• Molecular Weight: 17,623 Da
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: Igf1
• UniProt Synonym Gene Names: Igf-1; IGF-I
• UniProt Entry Name: IGF1_MOUSE