Application Data
(Proliferation assay with the mouse hybridoma cell line B9. The cells were stimulated with increasing amounts of recombinant mouse IL-6.)
IL-6 active protein
Mouse IL-6
Gene Names
Il6; Il-6
Reactivity
Mouse
Purity
> 98% by SDS-PAGE & Coomassie Stain
Synonyms
IL-6; N/A; Mouse IL-6; Recombinant Murine IL-6; IL-6 active protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 98% by SDS-PAGE & Coomassie Stain
Form/Format
Lyophilized
Sequence
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKEL CNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI S SGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDL HKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TL RSTRQT
Sequence Length
211
Endotoxin Level
< 0.1 ng /ug of protein (<1EU/ug)
Length (aa)
188
Result by N-terminal sequencing
M F P T S
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 50 mM acetic acid to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer (e.g. PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Biological Activity
The ED50 as determined by the dose-dependent
stimulation of murine hybridoma B9 cells is in the range of 5-20 pg/ml.
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
Application Data
(Proliferation assay with the mouse hybridoma cell line B9. The cells were stimulated with increasing amounts of recombinant mouse IL-6.)
Application Data
(Proliferation assay with the mouse hybridoma cell line B9. The cells were stimulated with increasing amounts of recombinant mouse IL-6.)
SDS-PAGE
(SDS-PAGE analysis of recombinant mouse IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.)
SDS-PAGE
(SDS-PAGE analysis of recombinant mouse IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.)
Related Product Information for IL-6 active protein
IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 188 amino acid residues.
Product Categories/Family for IL-6 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21.7 kDa
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
Il-6
NCBI Protein Information
interleukin-6; interleukin HP-1; B-cell hybridoma growth factor
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_MOUSE
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Product Notes
The IL-6 il6 (Catalog #AAA79260) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Mouse IL-6 reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKEL CNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI S SGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDL HKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TL RSTRQT. It is sometimes possible for the material contained within the vial of "IL-6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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