TNF-alpha active protein
Mouse TNF-alpha
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
Reactivity
Mouse
Purity
> 98% (SDS-PAGE; HPLC analyses
Synonyms
TNF-alpha; N/A; Mouse TNF-alpha; Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha active protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 98% (SDS-PAGE; HPLC analyses
Form/Format
Lyophilized
Sequence
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKD NQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK V NLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVN LPK YLDFAESGQV YFGVIAL
Sequence Length
156
Endotoxin level
< 0.1 ng/ug of protein (<1EU/ug).
Biological Activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < =0.1 ng/ml, corresponding to a specific activity of >=1 x 10^7 units/mg.
Length
156
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not votex. This solution can be stored at 2 degree C to 8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 12 months at -20 degree C to -80 degree C.
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Related Product Information for TNF-alpha active protein
TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-alpha is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Product Categories/Family for TNF-alpha active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.3 kDa
NCBI Official Full Name
tumor necrosis factor isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
Tnf
NCBI Official Synonym Symbols
DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
NCBI Protein Information
tumor necrosis factor; cachectin; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
Tnf
UniProt Synonym Gene Names
Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_MOUSE
Similar Products
Product Notes
The TNF-alpha tnf (Catalog #AAA79194) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Mouse TNF-alpha reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKD NQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK V NLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVN LPK YLDFAESGQV YFGVIAL. It is sometimes possible for the material contained within the vial of "TNF-alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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