Soluble RANK ligand Active Protein | RANK active protein

Mouse Soluble RANK ligand Recombinant

Cat. Num. AAA76384

• Gene Names: Tnfsf11; ODF; OPG; OPGL; RANKL; Ly109l; Trance
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Soluble RANK ligand; N/A; Mouse Soluble RANK ligand Recombinant; RANK active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse RANK Ligand was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MRRASRDYGK YLRSSEEMGS GPGVPHEGPL HPAPSAPAPA PPPAASRSMF LALLGLGLGQ VVCSIALFLY FRAQMDPNRI SEDSTHCFYR ILRLHENAGL QDSTLESEDT LPDSCRRMKQ AFQGAVQKEL QHIVGPQRFS GAPAMMEGSW LDVAQRGKPE AQPFAHLTIN AASIPSGSHK VTLSSWYHDR GWAKISNMTL SNGKLRVNQD GFYYLYANIC FRHHETSGSV PTDYLQLMVY VVKTSIKIPS SHNLMKGGST KNWSGNSEFH FYSINVGGFF KLRAGEEISI QVSNPSLLDP DQDATYFGAF KVQDID

• Source: Optimized DNA sequence encoding MouseRANK LigandTNF binding domainwas expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) determined by the dose-dependent stimulation of IL-8 production in Human PBMCs is <=8 ng/ml.
• Method: Osteoblast, osteoclast and macrophage differentiation.Bone marrow derived monocytes were seeded in 24-well culture plates and induced to differentiate into macrophages or osteoclasts via the addition of M-CSF or M-CSF + RANKL respectively.Osteoclastogenesis assaysOsteoclastogenesis assays were performed as in 5 cells were plated in each well of a 24-well dish with 60 ng/mL RANKL for 3 days.; After 3 days, 2×103 MDA-231, shControl, or shEGFR-MDA-231 cells were plated with the bone marrow cells, with 60 ng/mL RANKL and 10 ng/mL MCSF and grown for 3 days before TRAP staining.; For TRAP staining, cells were washed with 1×PBS, fixed with ice cold methanol for 10 minutes, and stained in fresh TRAP solution for 15 minutes at 37 degree C.; TRAP solution was replaced with 1×PBS for cell counting under the microscope.Osteoclastogenesis assaysOsteoclastogenesis assays were performed as in 5 cells were plated in each well of a 24-well dish with 60 ng/mL RANKL for 3 days.; After 3 days, 2×103 MDA-231, shControl, or shEGFR-MDA-231 cells were plated with the bone marrow cells, with 60 ng/mL RANKL and 10 ng/mL MCSF and grown for 3 days before TRAP staining.; For TRAP staining, cells were washed with 1×PBS, fixed with ice cold methanol for 10 minutes, and stained in fresh TRAP solution for 15 minutes at 37 degree C.; TRAP solution was replaced with 1×PBS for cell counting under the microscope.Osteoclast differentiation assayOsteoclast differentiation assay was performed as we described.+ T cells or Treg cells (1 × 106/ml) were pre-activated with 2.5 ug/ml anti-CD3 and 1.25 ug/ml anti-CD28 monoclonal mouse antibodies for 12 h as we described.
• Molecular Function: Cytokine, Developmental-protein, Receptor
• Biological Process: Differentiation
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(Polarized membrane extensions mediate osteoclast fusion.(D, top) Immunoblot analysis of lysates of RAW264.7 macrophages stimulated with RANKL for the indicated times with antibodies to Ser473-phosphorylated (p) or total forms of Akt.)

Application Data

(Expression of pro-resorptive cytokines in gouty tophus tissues.(c) Immunofluorescent stains for CD3 (green) and RANKL (red) in tophaceous gout tissues.)

Application Data

(PLC?2 is required for in vitro osteoclast development.(a) Representative TRAP-stained images of wild-type and PLC?2?/? bone marrow cells cultured in the presence of the indicated concentrations of recombinant murine M-CSF and RANKL for 4 days.)

Product Categories/Family for RANK active protein

Cytokines; TNF family

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NM_011613.3
• UniProt Accession #: O35235; O08712 TR11B_MOUSE; O35235 TNF11_MOUSE; O35305; P46414
• Molecular Weight: 35,003 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPG; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE