MIP-3 beta (CCL19) Active Protein | MIP-3beta active protein

Mouse MIP-3 beta (CCL19) Recombinant

Cat. Num. AAA76385

• Gene Names: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: MIP-3 beta (CCL19); N/A; Mouse MIP-3 beta (CCL19) Recombinant; MIP-3beta active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CCL19 was lyophilized from a.2 um filteredmM PB,100mM NaCl solution pH.5.
• Sequence: MAPRVTPLLA FSLLVLWTFP APTLGGANDA EDCCLSVTQR PIPGNIVKAF RYLLNEDGCR VPAVVFTTLR GYQLCAPPDQ PWVDRIIRRL KKSSAKNKGN STRRSPVS

• Source: Optimized DNA sequence encoding Mouse MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/ug (1EU/ug).
• Biological Activity: Determined by its ability to chemoattract human dendritic cells using a concentration range of.0-50.0 ng/ml.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at degree - degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(CyaA accelerates cell detachment and migration of TLR-activated DCs.Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.)

Application Data

(Effect of hematopoietic S1P lyase deficiency on T-cell proliferation and migration.*P)

Application Data

(Differential activation of MAP kinases in O. tsutsugamushi-infected DCs upon exposure to CCL19.DCs were stimulated with O. tsutsugamushi , or LPS for 18 h and then further incubated with CCL19 (200 ng/ml) for the indicated times.)

Application Data

(Analysis of chemokine receptors and chemotaxis.The migration of MSCs to different concentrations of CXCL12 and CCL19 was evaluated by chemotaxis assay.)

Application Data

Product Categories/Family for MIP-3beta active protein

Recombinant Human Cytokines; CCL chemokines

NCBI and Uniprot Product Information

• NCBI GI #: 6755422
• NCBI GeneID: 24047
• NCBI Accession #: NP_036018.1
• NCBI GenBank Nucleotide #: NM_011888.2
• UniProt Accession #: O70460
• Molecular Weight: 11,911 Da
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: Ccl19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; Scya19; exodus-3
• NCBI Protein Information: C-C motif chemokine 19; EBI-1 ligand chemokine; EBI1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; chemokine CCL19; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine A19; small-inducible c
• UniProt Protein Name: C-C motif chemokine 19
• UniProt Gene Name: Ccl19
• UniProt Synonym Gene Names: Elc; Scya19; EBI1 ligand chemokine; ELC
• UniProt Entry Name: CCL19_MOUSE