FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MIP-3 Beta/CCL19 antibody (AAA127069).Overlay histogram showing THP-1 cells stained with AAA127069 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MIP-3 Beta/CCL19 Antibody (AAA127069, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human MIP-3 Beta/CCL19 Polyclonal Antibody | anti-MIP-3Beta/CCL19 antibody
Anti-MIP-3 Beta/CCL19 Antibody
Gene Names
CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
MIP-3 Beta/CCL19, Antibody; Anti-MIP-3 Beta/CCL19 Antibody; Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody; anti-MIP-3Beta/CCL19 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Expressed in all tissues.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-MIP-3Beta/CCL19 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
E coli-derived human MIP-3 Beta/CCL19 recombinant protein (Position: G22-S98).
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MIP-3 Beta/CCL19 antibody (AAA127069).Overlay histogram showing THP-1 cells stained with AAA127069 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MIP-3 Beta/CCL19 Antibody (AAA127069, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-MIP-3 Beta/CCL19 antibody (AAA127069).Overlay histogram showing THP-1 cells stained with AAA127069 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MIP-3 Beta/CCL19 Antibody (AAA127069, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of MIP-3 Beta/CCL19 using anti-MIP-3 Beta/CCL19 antibody (AAA127069).MIP-3 Beta/CCL19 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIP-3 Beta/CCL19 Antibody (AAA127069) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of MIP-3 Beta/CCL19 using anti-MIP-3 Beta/CCL19 antibody (AAA127069).MIP-3 Beta/CCL19 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIP-3 Beta/CCL19 Antibody (AAA127069) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Product Categories/Family for anti-MIP-3Beta/CCL19 antibody
References
1. Yoshida R, Imai T, Hieshima K, Kusuda J, Baba M, Kitaura M, Nishimura M, Kakizaki M, Nomiyama H, Yoshie O (Jul 1997). "Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7". J Biol Chem. 272 (21): 13803-9.2. "Entrez Gene: CCL19 chemokine (C-C motif) ligand 19".
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,993 Da
NCBI Official Full Name
macrophage inflammatory protein 3 beta
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 19
NCBI Official Symbol
CCL19
NCBI Official Synonym Symbols
ELC; CKb11; MIP3B; MIP-3b; SCYA19
NCBI Protein Information
C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macroph
UniProt Protein Name
C-C motif chemokine 19
UniProt Gene Name
CCL19
UniProt Synonym Gene Names
ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta
UniProt Entry Name
CCL19_HUMAN
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Product Notes
The MIP-3Beta/CCL19 ccl19 (Catalog #AAA127069) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MIP-3 Beta/CCL19 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIP-3 Beta/CCL19 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MIP-3Beta/CCL19 ccl19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIP-3 Beta/CCL19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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