SDF-1 alpha Active Protein | SDF-1alpha active protein

Mouse SDF-1 alpha Recombinant

Cat. Num. AAA76395

• Gene Names: Cxcl12; Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: SDF-1 alpha; N/A; Mouse SDF-1 alpha Recombinant; SDF-1alpha active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant SDF-1 alpha was lyophilized from a 0.2 um filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
• Sequence: MDAKVVAVLA LVLAALCISD GKPVSLSYRC PCRFFESHIA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNK

• Source: Optimized DNA sequence encoding Mouse SDF-1 alpha mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of-40 ng/ml.
• Method: Cell MigrationThe upper chamber wells of CIM-Plates 16 were precoated with fibronectin, added to wells at 10 ug/ml (30 ul) and removed after 30 min.; The lower chamber wells were filled with 160 ul culture medium, allowing a clearly-defined meniscus to form.; SDF1, MCP1 and TNFalpha were added to the lower chambers at a final concentration of 100 ng/ml.; CIM-Plates were equilibrated for at least 30 min in a culture hood at room temperature.; Trypsin-EDTA-dispersed MPCs (100 ul in complete medium) were then plated in the fibronectin-precoated upper chamber wells at 306×103; cells/cm2 (600000 cells/ml) in 100 ul complete medium, which we determined as the optimal density to ensure confluent monolayers.The CIM-Plates were inserted into the cradle pockets of the RTCA DP; Analyzer, and the background impedance of each well was measured after 1 h. The RTCA DP Analyzer, placed in a standard cell-culture incubator, monitored the migrated cell number in each lower chamber well every 15 min (quadruplicate measurements) over 36 h. Data were analyzed with the RTCA Software.
• Molecular Function: Cytokine, Growth-factor
• Biological Process: Chemotaxis
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(Expression and function of the CXCR4 receptor in MET-depleted cell.(b) Western blot analysis of AKT and MAPK activation in shMET RH30 cells showed a decrease in the phosphorylation of these kinases following stimulation with human HGF (20?ng/ml) and SDF-1 (10?ng/ml and 100?ng/ml).)

Application Data

(The expression of the ?lobe A and 3A mutant fails to restore motility and polarity in BW5147?–?– cells.BW5147?–?– cells stably expressing WT or mutant DOCK2 (V1538A, ?lobe A, or 3A) were stimulated in suspension with CXCL12, and stained with phalloidin and DAPI.)

Application Data

(WHIM mutation CXCR4R334X increases CXCL12-induced calcium flux response, but allows homologous desensitization: analysis of transfected cells.)

Product Categories/Family for SDF-1alpha active protein

Recombinant Mouse Cytokines; CXC chemokines

NCBI and Uniprot Product Information

• NCBI GI #: 7305465
• NCBI GeneID: 20315
• NCBI Accession #: NP_038683.1
• NCBI GenBank Nucleotide #: NM_013655.4
• UniProt Accession #: P40224; Q8JLJ0; Q8QMN0; Q9JFT2
• Molecular Weight: 10,032 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: Cxcl12
• NCBI Official Synonym Symbols: Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• NCBI Protein Information: stromal cell-derived factor 1
• UniProt Protein Name: Stromal cell-derived factor 1
• UniProt Gene Name: Cxcl12
• UniProt Synonym Gene Names: Sdf1; SDF-1; TPAR1; PBSF; TLSF
• UniProt Entry Name: SDF1_MOUSE