Superoxide Dismutase 3, Extracellular Active Protein | SOD3 active protein

Active Superoxide Dismutase 3, Extracellular (SOD3)

Cat. Num. AAA21162

• Gene Names: SOD3; EC-SOD
• Reactivity: Homo sapiens (Human)
• Applications: Cell culture; Activity Assays.
• Purity: >90%
• Synonyms: Superoxide Dismutase 3, Extracellular; N/A; Active Superoxide Dismutase 3, Extracellular (SOD3); SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn; SOD3 active protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >90%
• Form/Format: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 800ug/mL (varies by lot)

• Applicable Applications for SOD3 active protein: Cell culture; Activity Assays.
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression.
• Residues: Trp19~Ala240
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 6.3
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-PAGE

(Sample: Active recombinant SOD3, Human)

WB (Western Blot)

(Western BlotSample: Recombinant SOD3, Human;Antibody: Rabbit Anti-Human SOD3 Ab)

SDS-PAGE

(Sample: Active recombinant SOD3, Human)

SDS-PAGE

(Sample: Active recombinant SOD3, Human)

Gene Sequencing (Extract)

Activity

(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. According to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30L 50mmol/L pyrogallol to 900L 50mmol/L Tris-HCl in 1.5mL cuvette (1.0cm light path), rapidly mixing at 25, then read at 325nm (zero the spectrophotometer using 50mmol/L Tris-HCl), record the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900L 50mmol/L Tris-HCl, incubated for 20min at 25, then adding 30L 50mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.)

Sequence Information

Product Categories/Family for SOD3 active protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 108885292
• NCBI GeneID: 6649
• UniProt Accession #: P08294
• Molecular Weight: Predicted Molecular Mass: 27.9kDa; Accurate Molecular Mass: 33/30kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: Extracellular superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 3
• NCBI Official Symbol: SOD3
• NCBI Official Synonym Symbols: EC-SOD
• NCBI Protein Information: extracellular superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Extracellular superoxide dismutase [Cu-Zn]
• UniProt Gene Name: SOD3
• UniProt Synonym Gene Names: EC-SOD
• UniProt Entry Name: SODE_HUMAN