Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 ug/ml (100 ul/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL.)
TGF-alpha Active Protein | TGFA active protein
TGF-alpha Protein, Human, Recombinant
Gene Names
TGFA; TFGA
Purity
>90% as determined by SDS-PAGE
Synonyms
TGF-alpha; N/A; TGF-alpha Protein, Human, Recombinant; Human TGFA/TGF-alpha Protein; TFGA Protein, Human; TGFa Protein, Human; transforming growth factor alpha; TGFA active protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Val40-Ala89
Species
Human
Predicted N Terminal
Met
Tag
Native
Protein Construction
A DNA sequence encoding the human TGFa (P01135-1)(Val40-Ala89) was expressed and purified.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2ug/ml (100uL/well) can bind Human EGFR hFc. The EC50 of Human EGFR hFc is 80-500ng/mL.
2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5ng/mL.
2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 ug/ml (100 ul/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 ug/ml (100 ul/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL.)
Application Data
(Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.)
Application Data
(Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.)
SDS-PAGE
SDS-PAGE
Related Product Information for TGFA active protein
Background: The miR-137 served as a tumor suppressor in non-small cell lung cancer (NSCLC) and its suppressive effect is mediated by repressing TGFA expression. TGFA gene expression was significantly higher in tumor tissues compared to adjacent normal tissue and high TGFA gene expression strongly correlated with poor survival in patients with lung adenocarcinoma, and miR-374a suppresses lung adenocarcinoma cell proliferation and invasion via targeting TGFA gene expression. Transforming growth factor alpha (TGFA) is a well-characterized mammalian growth factor that might contribute to the development of Cleft lip and palate (CL/P).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,006 Da
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_HUMAN
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Product Notes
The TGFA tgfa (Catalog #AAA257972) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Val40-Ala8 9. It is sometimes possible for the material contained within the vial of "TGF-alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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