IF (Immunofluorescence)
(293T cells were stained with mouse anti-beta-tubulin antibody and detected with EGFP-rProtein A (Green).)
EGFP recombinant protein
EGFP-rProtein A
Purity
>98% by SDS-PAGE and HPLC
Synonyms
EGFP; N/A; EGFP-rProtein A; EGFP recombinant protein
Host
E. coli
Specificity
Under optimal conditions, 1 mg EGFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of EGFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
Purity/Purification
>98% by SDS-PAGE and HPLC
Form/Format
Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.
Sequence Length
241
Endotoxin
< 0.05 EU/mg
IgG Binding
>95%
Bioburden
No organisms detected
Preparation and Storage
Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(293T cells were stained with mouse anti-beta-tubulin antibody and detected with EGFP-rProtein A (Green).)
IF (Immunofluorescence)
(293T cells were stained with mouse anti-beta-tubulin antibody and detected with EGFP-rProtein A (Green).)
Related Product Information for EGFP recombinant protein
The unique, high quality recombinant EGFP-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). EGFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
eGFP
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Product Notes
The EGFP (Catalog #AAA267285) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EGFP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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