Application Data
(The following figures demonstrate typical fluorescence rEGFP as tested in the GFP Fluorometric Quantitation Kit . Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff. One should use the data below for reference only. This data should not be used to interpret actual results.)
EGFP Recombinant Protein
Recombinant EGFP
Synonyms
EGFP; N/A; Recombinant EGFP; EGFP recombinant protein
Purity
Expressed and purified from E. coli as a recombinant protein, greater than 90% by SDS-PAGE (Figure 1). rEGFP has an approximate MW of 29 kDa, containing a 6xHis-tag at the C-terminus, and an excitation/emission spectra of 488/507 nm. Fluorescence activity was determined by GFP Fluorometric Quantitation Kit.
Quantity
100 ug/vial at 1.0 mg/mL in 1X PBS
Preparation and Storage
Store at -80°C; avoid freeze/thaw.
Shelf Life: 1 year from date of receipt under proper storage conditions
Shelf Life: 1 year from date of receipt under proper storage conditions
Application Data
(The following figures demonstrate typical fluorescence rEGFP as tested in the GFP Fluorometric Quantitation Kit . Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff. One should use the data below for reference only. This data should not be used to interpret actual results.)
Application Data
(The following figures demonstrate typical fluorescence rEGFP as tested in the GFP Fluorometric Quantitation Kit . Fluorescence measurement was performed on SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff. One should use the data below for reference only. This data should not be used to interpret actual results.)
Application Data
Application Data
Related Product Information for EGFP recombinant protein
• Includes 6xHis-tag at C-terminus
• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBS
Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in heterologous systems have established recombinant GFP (rGFP) as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. GFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the GFP molecule, many GFP variants have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rGFP requires no additional substrates or cofactors, rGFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.
• Provided at 100 ug/vial at 1.0 mg/mL in 1X PBS
Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in heterologous systems have established recombinant GFP (rGFP) as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. GFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the GFP molecule, many GFP variants have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rGFP requires no additional substrates or cofactors, rGFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.
Product Categories/Family for EGFP recombinant protein
References
1. Chalfie M. et al, Science 263: 802-805, 1994.
2. Cormack B.P. et al, Gene 173: 33-38, 1996.
3. Rizzuto R. et al, Curr.Biol. 6:183-188 1996.
1. Caschera, F. & Noireaux, V. (2015). Preparation of amino acid mixtures for cell-free expression systems. Biotechniques. 58:40-43.
2. Caschera, F. & Noireaux, V. (2015). A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system. Metab Eng. 27:29-37.
3. Sokolova, E. et al. (2013). Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate. PNAS. 110: 11692-11697.
2. Cormack B.P. et al, Gene 173: 33-38, 1996.
3. Rizzuto R. et al, Curr.Biol. 6:183-188 1996.
1. Caschera, F. & Noireaux, V. (2015). Preparation of amino acid mixtures for cell-free expression systems. Biotechniques. 58:40-43.
2. Caschera, F. & Noireaux, V. (2015). A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system. Metab Eng. 27:29-37.
3. Sokolova, E. et al. (2013). Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate. PNAS. 110: 11692-11697.
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Product Notes
The EGFP (Catalog #AAA11426) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EGFP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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