SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Neutrophil Elastase/ELA2 Recombinant Protein | ELA2 recombinant protein
Recombinant Neutrophil Elastase/ELA2
Gene Names
Elane; NE; Ela2; F430011M15Rik
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Neutrophil Elastase/ELA2; N/A; Recombinant Neutrophil Elastase/ELA2; Elane; Ela2; F430011M15Rik; NE; Neutrophil elastase; Elastase-2; Leukocyte elastase; ELA2 recombinant protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In 20mM Tris-HCl Buffer (pH 8.0) Containing 0.1M NaCl, 30% Glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
27-265aa
Sequence
SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAH DLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGD RTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPL VCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTN H HHHHH
Sequence Length
265
Applicable Applications for ELA2 recombinant protein
SDS-PAGE
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ser 27
Preparation and Storage
For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for ELA2 recombinant protein
Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E Coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for ELA2 recombinant protein
References
Wiesmeier M., et al, (2016) PLoS ONE 11:e0168055. Sturrock A., et al, (1998) Cytogenet. Cell Genet. 83:104-108.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.8kDa (245aa); 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
neutrophil elastase
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
Elane
NCBI Official Synonym Symbols
NE; Ela2; F430011M15Rik
NCBI Protein Information
neutrophil elastase
UniProt Protein Name
Neutrophil elastase
UniProt Gene Name
Elane
UniProt Synonym Gene Names
Ela2
UniProt Entry Name
ELNE_MOUSE
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Product Notes
The ELA2 elane (Catalog #AAA48315) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 27-265aa. AAA Biotech's Neutrophil Elastase/ELA2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ELA2 elane for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAH DLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGD RTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPL VCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTN H HHHHH. It is sometimes possible for the material contained within the vial of "Neutrophil Elastase/ELA2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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