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Deoxynivalenol (DON) ELISA Kit | DON elisa kit

Deoxynivalenol (DON) ELISA Kit

Gene Names
dj; bs02h03.y1; CG1980; Dj; DJ; DmelCG1980
Synonyms
Deoxynivalenol (DON); N/A; Deoxynivalenol (DON) ELISA Kit; DON elisa kit
Ordering
Sequence Length
187
Assay Type
Competitive
Samples
Feedstuff, feed, grain
Detection Range
1 ug/kg~81 ug/kg
Sensitivity
< 1 ug/kg
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for DON elisa kit
Background: Deoxynivalenol (DON), also known as Vomitoxin, belongs to trichothecene and is produced by mould fusarium, growing in grains such as wheat, maize, barley and stover. Because of causing vomitting in pigs, hence the name is Vomitoxin. It has some harmful effects to human body and has been listed as Category III carcinogen by EU classification criteria. In 2011, the limit of Deoxynivalenol residues is 1000 ug/kg in grains and grains products in China. This kit is a detection product developed based on ELISA technology, with operation time as short as 25 min and a sensitivity of 1 ug/kg, and linear range from 1 ug/kg~81 ug/kg.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Deoxynivalenol antigen. Standards or samples are added to the appropriate microtiter plate wells with Deoxynivalenol antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Deoxynivalenol and Deoxynivalenol in standards or samples with the Deoxynivalenol antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Deoxynivalenol in the standards or samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,521 Da
NCBI Official Full Name
don juan, isoform C
NCBI Official Synonym Full Names
don juan
NCBI Official Symbol
dj
NCBI Official Synonym Symbols
bs02h03.y1; CG1980; Dj; DJ; DmelCG1980
NCBI Protein Information
CG1980 gene product from transcript CG1980-RA
UniProt Protein Name
Sperm-specific protein Don juan
UniProt Gene Name
dj
UniProt Entry Name
DJ_DROME

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Product Notes

The DON dj (Catalog #AAA80519) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Deoxynivalenol (DON), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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