Application Data
(Figure 2: GST-Rhotekin in BL21 Bacterial Lysate. BL21 E.Coli lysate was first diluted to 0.2 mg/mL with bacterial sonication buffer (PBS/1% Triton X-100), then further dilute 1000 fold with Assay Diluent. Diluted E.Coli lysate was subjected to GST ELISA Kit according to the Assay Protocol.)
GST elisa kit
GST ELISA Kit
Synonyms
GST; N/A; GST ELISA Kit; GST elisa kit
Detection Method
Colorimetric
Principle of the Assay
The GST protein standards, and the unknown GST or GST-tagged protein samples, are first added to an anti-GST polyclonal antibody coated plate. After incubation, an FITC-conjugated anti-GST antibody is added, followed by an HRP-conjugated mouse anti-FITC antibody. The GST-tag protein content in unknown samples is determined by comparing with a standard curve that is prepared from GST protein standards.
Preparation and Storage
Upon receipt, aliquot and store the recombinant GST Standard at -20 degree C and avoid freeze/thaw. Store all other components at 4 degree.
Application Data
(Figure 2: GST-Rhotekin in BL21 Bacterial Lysate. BL21 E.Coli lysate was first diluted to 0.2 mg/mL with bacterial sonication buffer (PBS/1% Triton X-100), then further dilute 1000 fold with Assay Diluent. Diluted E.Coli lysate was subjected to GST ELISA Kit according to the Assay Protocol.)
Application Data
(Figure 2: GST-Rhotekin in BL21 Bacterial Lysate. BL21 E.Coli lysate was first diluted to 0.2 mg/mL with bacterial sonication buffer (PBS/1% Triton X-100), then further dilute 1000 fold with Assay Diluent. Diluted E.Coli lysate was subjected to GST ELISA Kit according to the Assay Protocol.)
Application Data
(The following figures demonstrate typical results with the GST ELISA Kit. One should use the data below for reference only. This data should not be used to interpret actual results.)
Application Data
(The following figures demonstrate typical results with the GST ELISA Kit. One should use the data below for reference only. This data should not be used to interpret actual results.)
Related Product Information for GST elisa kit
Glutathione S-transferase (GST) is a naturally-occurring 26 kDa protein found in eukaryotic cells. The gene from the parasitic helminth Schistosoma japonicum was used in the development of the GST affinity tag to aid in the purification of recombinant proteins. The 26 kDa GST moiety binds with high affinity to glutathione coupled to a matrix. This binding is reversible and the protein can be eluted under mild, non-denaturing conditions by the addition of reduced glutathione to the elution buffer. A specific protease site engineered between the GST moiety and the protein of interest allows removal of the GST moiety from the target recombinant protein. The GST can be removed from the sample by re-chromatography on a glutathione column, and the protein of interest purified to homogeneity by other techniques such as gel filtration or ion exchange. GST ELISA Kit is an enzyme immunoassay developed for detection and quantitation of GST or GST-tagged proteins in cell or tissue samples. The kit has detection sensitivity limit of 78 pg/mL GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST samples.
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Product Notes
The GST (Catalog #AAA44711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GST, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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