Chicken Epithelial neutrophil activating peptide 78 ELISA Kit | ENA-78 elisa kit

Chicken Epithelial neutrophil activating peptide 78 ELISA Kit

Cat. Num. AAA91845

• Gene Names: CXCL5; SCYB5; ENA-78
• Reactivity: Chicken
• Synonyms: Epithelial neutrophil activating peptide 78; N/A; Chicken Epithelial neutrophil activating peptide 78 ELISA Kit; ENA-78 elisa kit

• Reactivity: Chicken
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.

• Intended Uses: This ENA-78 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ENA-78. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for ENA-78 elisa kit

Principle of the Assay: ENA-78 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ENA-78 antibody and an ENA-78-HRP conjugate. The assay sample and buffer are incubated together with ENA-78-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENA-78 concentration since ENA-78 from samples and ENA-78-HRP conjugate compete for the anti-ENA-78 antibody binding site. Since the number of sites is limited, as more sites are occupied by ENA-78 from the sample, fewer sites are left to bind ENA-78-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENA-78 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ENA-78 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 471243
• NCBI GeneID: 6374
• UniProt Accession #: P42830; Q96QE1; Q6I9S7
• Molecular Weight: 11,972 Da
• NCBI Official Full Name: ENA-78
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 5
• NCBI Official Symbol: CXCL5
• NCBI Official Synonym Symbols: SCYB5; ENA-78
• NCBI Protein Information: C-X-C motif chemokine 5; ENA-78(1-78); small inducible cytokine B5; small-inducible cytokine B5; neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; epithelial-derived neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
• UniProt Protein Name: C-X-C motif chemokine 5
• UniProt Gene Name: CXCL5
• UniProt Synonym Gene Names: ENA78; SCYB5
• UniProt Entry Name: CXCL5_HUMAN