Application Data
(HTRF assay for EPHB2 (570-987) activity 1 uM TK substrate was incubated with different concentrations of EPHB2 (570-987) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1 mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
EPHB2 recombinant protein
Recombinant EPHB2 (570-987) protein
Synonyms
EPHB2; N/A; Recombinant EPHB2 (570-987) protein; EPH receptor B2; EPHT3; HEK5; DRT; ERK; Hek5; Tyro5; EPHB2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant EPHB2 (570-987) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant EPHB2 (570-987) protein that includes amino acids 570-987 of human EPHB2 protein (accession number NP_004433.2) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 48.65kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for EPHB2 (570-987) activity 1 uM TK substrate was incubated with different concentrations of EPHB2 (570-987) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1 mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for EPHB2 (570-987) activity 1 uM TK substrate was incubated with different concentrations of EPHB2 (570-987) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1 mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for EPHB2 (570-987) activity 1 uM TK substrate was incubated with different concentrations of EPHB2 (570-987) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1 mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for EPHB2 (570-987) activity 1 uM TK substrate was incubated with different concentrations of EPHB2 (570-987) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1 mM MnCl2, 1 mM DTT, 5nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant EPHB2 (570-987) protein 10% SDS-PAGE Coomassie staining MW: 48.65kDa Purity: >=95%)
SDS-PAGE
(Recombinant EPHB2 (570-987) protein 10% SDS-PAGE Coomassie staining MW: 48.65kDa Purity: >=95%)
Related Product Information for EPHB2 recombinant protein
EPHB2 is a receptor tyrosine kinase,has a prototypical RTK topology including an N-terminal multidomain extracellular region, a membrane spanning region, and an intracellular region.EPHB2 binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.
Product Categories/Family for EPHB2 recombinant protein
Similar Products
Product Notes
The EPHB2 (Catalog #AAA60390) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EPHB2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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