Thrombin Recombinant Protein | F2 recombinant protein
Recombinant Human Thrombin, HEK
Gene Names
F2; PT; THPH1; RPRGL2
Synonyms
Thrombin; N/A; Recombinant Human Thrombin, HEK; Thrombin Human, HEK; Thrombin Human Recombinant, HEK; Prothrombin; EC 3.4.21.5; Coagulation factor II; F2; PT; THPH1; RPRGL2; Thrombin, HEK; F2 recombinant protein
Host
HEK (HumaXpress)
Form/Format
The Thrombin solution (1mg/ml) contains 20mM MES, pH6.0 and 500mM Choline Chloride.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence Length
622
Enzyme Assay
Thrombin (1nM) was assayed using FPRpNA as a substrate (20uM) in 5mM Tris-HCl (pH8.0), 0.1% PEG, 200mM NaCl at 25 degree C and monitored at 405 nm.
Preparation and Storage
Store frozen at -20 degree C to -80 degree C for long periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for F2 recombinant protein
Description: Recombinant Human Thrombin produced in HEK cells, having a total molecular weight of 36kDa. The Thrombin is purified by proprietary chromatographic techniques.
Introduction: Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
Introduction: Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
Product Categories/Family for F2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,037 Da
NCBI Official Full Name
prothrombin preproprotein
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
NCBI Protein Information
prothrombin; prepro-coagulation factor II; prothrombin B-chain; serine protease
UniProt Protein Name
Prothrombin
UniProt Gene Name
F2
UniProt Entry Name
THRB_HUMAN
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Product Notes
The F2 f2 (Catalog #AAA39049) is a Recombinant Protein produced from HEK (HumaXpress) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thrombin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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