Recombinant Protein
Tips and technical resources to kickstart your experiments and keep them moving forward, including concentration calculations, centrifuges, sample management, and more.
A recombinant protein is produced by inserting a gene of interest into an expression system, resulting in a lab-grown protein outside its natural environment.
It means the protein was generated by genetic engineering, using host cells to express a gene not native to that cell line or organism.
Recombinant proteins are engineered and produced in lab systems; natural proteins originate from their native biological animal/cell sources and may vary in purity and composition.
This approach enables controlled, large-scale production, consistency across batches, and the generation of proteins not easily sourced from nature.
It involves gene cloning, transformation into host cells, expression induction, purification with tags, and validation for purity and activity.
They offer high purities, defined sequences, customizable tags, broad species coverage, and transparency in validation - enabling reproducible and scalable experiments.
Recombinant proteins are commonly produced in bacterial (E. coli), yeast, mammalian, and insect cell systems. AAA Biotech’s catalog includes proteins expressed with tags like FLAG, GST, His, and MBP for various research needs.
Recombinant proteins allow researchers to study proteins in isolation, produce large quantities, and investigate disease mechanisms or drug interactions without relying on animal- or tissue-derived sources.
They’re purified using chromatography methods, often with affinity tags (e.g., His-tag) and SDS-PAGE validation. AAA Biotech’s offerings feature proteins with >90% purity for high experimental fidelity.
Yes, improper folding, harsh purification, or repeated freeze-thaw cycles can reduce activity. Optimal buffer composition and storage at recommended temperatures help maintain integrity.
Recombinant proteins are used as standards in ELISA kits, antigens in immunoassays, and controls for molecular detection due to their defined purity and batch consistency.
No, not all are suitable; therapeutic-grade proteins require rigorous validation of purity, activity, and absence of contaminants, beyond typical research-use standards.
Activity is confirmed using functional bioassays specific to the protein, such as enzymatic activity tests, ligand binding, or cell-based assays, and sometimes compared to native protein controls.
Quality-focused providers minimize these issues, but slight variations may occur due to expression system and purification process differences. AAA Biotech places extremely high value on validated, consistent batches.
Many vaccines rely on recombinant proteins as antigens to trigger immune responses, allowing safer, scalable, production without using live pathogens.
Proteins can generally be stored for months to a few years at -20°C or lower, but stability depends on protein type and formulation. Degradation risk increases with improper storage.
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