Standard Curve (Sample)
Human CD95 ELISA Kit | FAS elisa kit
Human CD95 ELISA Kit
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
ELISA
Synonyms
CD95; N/A; Human CD95 ELISA Kit; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; FAS elisa kit
Reactivity
Human
Specificity
The Human CD95 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD95 proteins within the range of 125 pg/ml - 8000 pg/ml.
Sequence Length
335
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
62 pg/ml.
Applicable Applications for FAS elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FAS elisa kit
Principle of the Assay||The Human CD95 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD95 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD95 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD95 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD95 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD95 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48 kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.
Background/Introduction: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48 kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,781 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 6
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
FAS
NCBI Official Synonym Symbols
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6
UniProt Gene Name
FAS
UniProt Synonym Gene Names
APT1; FAS1; TNFRSF6
UniProt Entry Name
TNR6_HUMAN
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Product Notes
The Human FAS fas (Catalog #AAA261703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261703 ELISA Kit recognizes Human FAS. AAA Biotech's CD95 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the FAS fas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD95, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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