Human FASL ELISA Kit | FASLG elisa kit

Human FASL PicoKine ELISA Kit

Cat. Num. AAA45342

• Gene Names: Fasl; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Reactivity: Human
• Synonyms: FASL; N/A; Human FASL PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 6; Fas ligand (TNF superfamily, member 6); Apoptosis (APO 1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; CD95L protein; Fas antigen ligand; FASLG; TNF superfamily member 6; TNFSF6; Tumor necrosis factor (ligand) superfamily member 6; FASLG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human FASL
• Sequence Length: 131
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 2pg/ml

• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human FASL PicoKine ELISA Kit standard curve)

Related Product Information for FASLG elisa kit

Principle of the assay: human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards (CHO,P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.
Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

NCBI and Uniprot Product Information

• NCBI GI #: 81903309
• NCBI GeneID: 14103
• UniProt Accession #: Q99PH8; P41047
• Molecular Weight: 14,538 Da
• NCBI Official Full Name: FasL isoform
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: Fasl
• NCBI Official Synonym Symbols: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease
• UniProt Protein Name: FasL isoform
• UniProt Gene Name: Fasl
• UniProt Entry Name: Q99PH8_MOUSE