Human Fibulin 5 ELISA Kit | FBLN5 elisa kit

Human Fibulin 5 ELISA Kit

Cat. Num. AAA16416

• Gene Names: FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
• Reactivity: Human
• Synonyms: Fibulin 5; N/A; Human Fibulin 5 ELISA Kit; FBLN5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FBLN5. No significant cross-reactivity or interference between FBLN5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBLN5 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for FBLN5 elisa kit

Principle of the Assay: FBLN5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FBLN5. Standards or samples are then added to the microtiter plate wells and FBLN5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FBLN5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FBLN5 are added to each well to "sandwich" the FBLN5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FBLN5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBLN5 concentration in each sample is interpolated from this standard curve.

Intended Uses: This FBLN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FBLN5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for FBLN5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 19743803
• NCBI GeneID: 10516
• NCBI Accession #: NP_006320.2
• NCBI GenBank Nucleotide #: NM_006329.3
• Molecular Weight: 50,180 Da
• NCBI Official Full Name: fibulin-5
• NCBI Official Synonym Full Names: fibulin 5
• NCBI Official Symbol: FBLN5
• NCBI Official Synonym Symbols: EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
• NCBI Protein Information: fibulin-5; urine p50 protein; developmental arteries and neural crest EGF-like protein
• UniProt Protein Name: Fibulin-5
• UniProt Gene Name: FBLN5
• UniProt Synonym Gene Names: DANCE; UNQ184/PRO210; FIBL-5; Dance; UP50
• UniProt Entry Name: FBLN5_HUMAN