Standard Curve (Sample)
Human Fibulin 5 (FBLN5) ELISA Kit | FBLN5 elisa kit
Human Fibulin 5 (FBLN5) ELISA Kit
Gene Names
FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
Reactivity
Human
Synonyms
Fibulin 5 (FBLN5); N/A; Human Fibulin 5 (FBLN5) ELISA Kit; ARMD3; DANCE; EVEC; UP50; FIBL-5; Dance; Developmental arteries and neural crest EGF-like protein; FBLN5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibulin 5 (FBLN5).
No significant cross-reactivity or interference between Fibulin 5 (FBLN5) and analogues was observed.
No significant cross-reactivity or interference between Fibulin 5 (FBLN5) and analogues was observed.
Sequence Length
448
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Urine and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
5.6pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Fibulin 5 (FBLN5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Fibulin 5 (FBLN5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FBLN5 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 5 (FBLN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 5 (FBLN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 5 (FBLN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibulin 5 (FBLN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FBLN5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,180 Da
NCBI Official Full Name
fibulin-5
NCBI Official Synonym Full Names
fibulin 5
NCBI Official Symbol
FBLN5
NCBI Official Synonym Symbols
EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
NCBI Protein Information
fibulin-5
UniProt Protein Name
Fibulin-5
UniProt Gene Name
FBLN5
UniProt Synonym Gene Names
DANCE; FIBL-5; Dance; UP50
UniProt Entry Name
FBLN5_HUMAN
Similar Products
Product Notes
The Human FBLN5 fbln5 (Catalog #AAA20739) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA20739 ELISA Kit recognizes Human FBLN5. It is sometimes possible for the material contained within the vial of "Fibulin 5 (FBLN5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.