Fc gamma RIIIA/CD16a Recombinant Protein | FcgammaRIIIA/CD16a recombinant protein

Recombinant human Fc gamma RIIIA/CD16a protein

Cat. Num. AAA48425

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Fc gamma RIIIA/CD16a; N/A; Recombinant human Fc gamma RIIIA/CD16a protein; Low affinity immunoglobulin gamma Fc region receptor III-A;low affinity immunoglobulin gamma Fc region receptor III-A isoform c; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA; FcR-10; IgG Fc receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; FCGRIII; IGFR3; IMD20; CD16; FcgammaRIIIA/CD16a recombinant protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-208aa
• Sequence: MRTEDLPKAV VFLEPQWYRV LEKDSVTLKC QGAYSPEDNS TQWFHNESLI SSQASSYFID AATVDDSGEY RCQTNLSTLS DPVQLEVHIG WLLLQAPRWV FKEEDPIHLR CHSWKNTALH KVTYLQNGKG RKYFHHNSDF YIPKATLKDS GSYFCRGLFG SKNVSSETVN ITITQGLAVS TISSFFPPGY Q

• Applicable Applications for FcgammaRIIIA/CD16a recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: His-Tag
• Predicted Nterm: Met 18
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its binding ability in a functional ELISA with Human IgG1 Fc.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human Fc gamma RIIIA/CD16a is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc in a functional ELISA assay.)

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for FcgammaRIIIA/CD16a recombinant protein

Fc gamma RIIIA/CD16a is also known as Low affinity immunoglobulin gamma Fc region receptor III-A isoform c. It is optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Also mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC. Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis. Fc gamma RIIIA is mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells. Recombinant human Fc gamma RIIIA/CD16a, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for FcgammaRIIIA/CD16a recombinant protein

Immunology

References

Lee J., et al, (2015) Immunity. 42(3):431-442.;; DiLillo D.J., et al, (2014) Nat Med. 20(2):143-151.;; Warren H.S., et al, (1999) J Immunol. 162(2):735-742.;; Grier J.T., et al, (2012) J Clin Invest. 122(10):3769-3780.

NCBI and Uniprot Product Information

• UniProt Accession #: P08637
• Molecular Weight: 22.6kDa (197aa)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
• UniProt Entry Name: FCG3A_HUMAN