Human Ficolin-3 ELISA Kit | FCN3 elisa kit

Human Ficolin-3 PicoKine ELISA Kit

Cat. Num. AAA45791

• Gene Names: FCN3; FCNH; HAKA1
• Reactivity: Human
• Synonyms: Ficolin-3; N/A; Human Ficolin-3 PicoKine ELISA Kit; ficolin (collagen/fibrinogen domain containing) 3; Collagen/fibrinogen domain containing lectin 3 p35; Collagen/fibrinogen domain containing protein 3; FCN 3; FCNH; Ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen); Ficolin (collagen/fibrinogen domain containing) 3; Ficolin 3; H ficolin; HAKA1; Hakata antigen; MGC22543; FCN3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Ficolin-3
• Sequence Length: 299
• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: < 20pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Ficolin-3 PicoKine ELISA Kit standard curve)

Related Product Information for FCN3 elisa kit

Principle of the assay: human Ficolin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been precoated onto 96-well plates. Standards(NSO, Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-3 amount of sample captured in plate.
Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 90111858
• NCBI GeneID: 8547
• UniProt Accession #: O75636; Q6IBJ5; Q8WW86
• Molecular Weight: 31,678 Da
• NCBI Official Full Name: Ficolin-3
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 3
• NCBI Official Symbol: FCN3
• NCBI Official Synonym Symbols: FCNH; HAKA1
• NCBI Protein Information: ficolin-3; H-ficolin; ficolin 3; collagen/fibrinogen domain-containing protein 3; collagen/fibrinogen domain-containing lectin 3 p35; ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
• UniProt Protein Name: Ficolin-3
• UniProt Gene Name: FCN3
• UniProt Synonym Gene Names: FCNH; HAKA1
• UniProt Entry Name: FCN3_HUMAN