FCM/FACS (Flow Cytometry)
(FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human FCN3 Polyclonal Antibody | anti-FCN3 antibody
FCN3 Antibody (C-term)
Gene Names
FCN3; FCNH; HAKA1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FCN3, Antibody; FCN3 Antibody (C-term); Ficolin-3; Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; Hakata antigen; FCN3; FCNH; HAKA1; anti-FCN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 amino acids from the C-terminal region of human FCN3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.27 (varies by lot)
Sequence Positions
214-243
Sequence Length
288
Applicable Applications for anti-FCN3 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(FCN3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(FCN3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(FCN3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).)
WB (Western Blot)
(FCN3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).)
Related Product Information for anti-FCN3 antibody
Ficolins are a group of proteins which consist of a
collagen-like domain and a fibrinogen-like domain. In human serum,
there are two types of ficolins, both of which have lectin
activity. The protein encoded by this gene is a thermolabile
beta-2-macroglycoprotein found in all human serum and is a member
of the ficolin/opsonin p35 lectin family. The protein, which was
initially identified based on its reactivity with sera from
patients with systemic lupus erythematosus, has been shown to have
a calcium-independent lectin activity. The protein can activate the
complement pathway in association with MASPs and sMAP, thereby
aiding in host defense through the activation of the lectin
pathway. Alternative splicing occurs at this locus and two
variants, each encoding a distinct isoform, have been identified.
collagen-like domain and a fibrinogen-like domain. In human serum,
there are two types of ficolins, both of which have lectin
activity. The protein encoded by this gene is a thermolabile
beta-2-macroglycoprotein found in all human serum and is a member
of the ficolin/opsonin p35 lectin family. The protein, which was
initially identified based on its reactivity with sera from
patients with systemic lupus erythematosus, has been shown to have
a calcium-independent lectin activity. The protein can activate the
complement pathway in association with MASPs and sMAP, thereby
aiding in host defense through the activation of the lectin
pathway. Alternative splicing occurs at this locus and two
variants, each encoding a distinct isoform, have been identified.
Product Categories/Family for anti-FCN3 antibody
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Andersen, T., et al. J. Rheumatol. 36(4):757-759(2009)
Ruskamp, J.M., et al. Clin. Exp. Immunol. 155(3):433-440(2009)
Lacroix, M., et al. J. Immunol. 182(1):456-465(2009)
Munthe-Fog, L., et al. Mol. Immunol. 45(9):2660-2666(2008)
Andersen, T., et al. J. Rheumatol. 36(4):757-759(2009)
Ruskamp, J.M., et al. Clin. Exp. Immunol. 155(3):433-440(2009)
Lacroix, M., et al. J. Immunol. 182(1):456-465(2009)
Munthe-Fog, L., et al. Mol. Immunol. 45(9):2660-2666(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32903
NCBI Official Full Name
ficolin-3 isoform 1
NCBI Official Synonym Full Names
ficolin (collagen/fibrinogen domain containing) 3
NCBI Official Symbol
FCN3
NCBI Official Synonym Symbols
FCNH; HAKA1
NCBI Protein Information
ficolin-3
UniProt Protein Name
Ficolin-3
UniProt Gene Name
FCN3
UniProt Synonym Gene Names
FCNH; HAKA1
UniProt Entry Name
FCN3_HUMAN
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Product Notes
The FCN3 fcn3 (Catalog #AAA290088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 214-243. The FCN3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FCN3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the FCN3 fcn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FCN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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