Standard Curve (Sample)
Fish Tumor Necrosis Factor alpha (TNF-alpha) ELISA Kit | TNF-alpha elisa kit
Fish Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Reactivity
Fish
Synonyms
Tumor Necrosis Factor alpha (TNF-alpha); N/A; Fish Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit; Fish Tumor Necrosis Factor alpha (TNF-alpha) ELISA KIT; TNF-alpha elisa kit
Reactivity
Fish
Specificity
This assay has high sensitivity and excellent specificity for detection of fish TNF-alpha. No significant cross-reactivity or interference between fish TNF-alpha and analogues was observed.
Sequence Length
233
Assay Type
Competitive
Samples
Serum, plasma
Detection Range
125 pg/ml-5000 pg/ml
Sensitivity
< 125pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNF-alpha elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for TNF-alpha and Horseradish Peroxidase (HRP) conjugated TNF-alpha. The competitive inhibition reaction is launched between with HRP labeled TNF-alpha and unlabeled TNF-alpha with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TNF-alpha in the sample. The color development is stopped and the intensity of the color is measured.
References
Aeromonas hydrophila-induced alterations in cytosolic calcium activate pro-apoptotic cPKC-MEK1/2-TNF? axis in infected headkidney macrophages of Clarias gariepinus. Asha Shelly, Chaitali Banerjee, Gunjan Kumar Saurav, Atish Ray, Vipin Singh Rana, Rajagopal Raman, Shibnath Mazumder (2017)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,644 Da
NCBI Official Full Name
TNF-alpha
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The Fish TNF-alpha tnf (Catalog #AAA15605) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15605 ELISA Kit recognizes Fish TNF-alpha. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor alpha (TNF-alpha), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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