Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human FLOT1. No significant cross-reactivity or interference between human FLOT1 and analogues was observed.
Sequence Length
427
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
12.5 pg/ml-800 pg/ml
Sensitivity
< 3.12 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for FLOT1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLOT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,355 Da
NCBI Official Full Name
flotillin-1
NCBI Official Synonym Full Names
flotillin 1
NCBI Official Symbol
FLOT1
NCBI Protein Information
flotillin-1; integral membrane component of caveolae
UniProt Protein Name
Flotillin-1
UniProt Gene Name
FLOT1
UniProt Entry Name
FLOT1_HUMAN