Standard Curve (Sample)
Rat Flotillin-1 (FLOT1) ELISA Kit | FLOT1 elisa kit
Rat Flotillin-1 (FLOT1) ELISA Kit
Reactivity
Rat
Synonyms
Flotillin-1 (FLOT1); N/A; Rat Flotillin-1 (FLOT1) ELISA Kit; FLOT1 elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FLOT1 elisa kit
Intended Uses: This FLOT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FLOT-1. This ELISA kit for research use only!
Principle of the Assay: FLOT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FLOT-1 antibody and an FLOT-1-HRP conjugate. The assay sample and buffer are incubated together with FLOT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FLOT-1 concentration since FLOT-1 from samples and FLOT-1-HRP conjugate compete for the anti-FLOT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FLOT-1 from the sample, fewer sites are left to bind FLOT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLOT-1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FLOT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FLOT-1 antibody and an FLOT-1-HRP conjugate. The assay sample and buffer are incubated together with FLOT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FLOT-1 concentration since FLOT-1 from samples and FLOT-1-HRP conjugate compete for the anti-FLOT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FLOT-1 from the sample, fewer sites are left to bind FLOT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLOT-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FLOT1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,355 Da
NCBI Official Full Name
flotillin-1
NCBI Official Synonym Full Names
flotillin 1
NCBI Official Symbol
FLOT1
NCBI Protein Information
flotillin-1; integral membrane component of caveolae
UniProt Protein Name
Flotillin-1
UniProt Gene Name
FLOT1
UniProt Entry Name
FLOT1_HUMAN
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Product Notes
The Rat FLOT1 flot1 (Catalog #AAA245359) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245359 ELISA Kit recognizes Rat FLOT1. It is sometimes possible for the material contained within the vial of "Flotillin-1 (FLOT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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