Standard Curve (Sample)
Human sVEGFR1/sFLT1 ELISA Kit | FLT1 elisa kit
Human sVEGFR1/sFLT1 PicoKine ELISA Kit
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Synonyms
sVEGFR1/sFLT1; N/A; Human sVEGFR1/sFLT1 PicoKine ELISA Kit; Vascular endothelial growth factor receptor 1; fms-related tyrosine kinase 1; FLT; FLT 1; Flt-1; FLT1; Fms like tyrosine kinase 1; Fms related tyrosine kinase 1; Fms-like tyrosine kinase 1; Tyrosine protein kinase FRT; Tyrosine protein kinase receptor FLT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular endothelial growth factor/vascular permeability factor receptor; Vascular permeability factor receptor; VEGFR 1; VEGFR-1; VEGFR1; VGFR1_HUMAN; FLT1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human FLT1
Sequence Length
1338
Samples
Cell culture supernatants, serum and plasma (EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<30 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FLT1 elisa kit
Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1 (sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
Principle of the Assay: The Picokine™ Human FLT1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human FLT1 with a 96-well strip plate that is pre-coated with antibody specific for FLT1. The detection antibody is a biotinylated antibody specific for FLT1. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human FLT1 protein as standards. To measure Human FLT1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human FLT1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FLT1 in the sample.
Principle of the Assay: The Picokine™ Human FLT1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human FLT1 with a 96-well strip plate that is pre-coated with antibody specific for FLT1. The detection antibody is a biotinylated antibody specific for FLT1. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human FLT1 protein as standards. To measure Human FLT1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human FLT1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FLT1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,175 Da
NCBI Official Full Name
Vascular endothelial growth factor receptor 1
NCBI Official Synonym Full Names
fms-related tyrosine kinase 1
NCBI Official Symbol
FLT1
NCBI Official Synonym Symbols
FLT; FLT-1; VEGFR1; VEGFR-1
NCBI Protein Information
vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
UniProt Protein Name
Vascular endothelial growth factor receptor 1
UniProt Gene Name
FLT1
UniProt Synonym Gene Names
FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
UniProt Entry Name
VGFR1_HUMAN
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Product Notes
The Human FLT1 flt1 (Catalog #AAA11542) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11542 ELISA Kit recognizes Human FLT1. It is sometimes possible for the material contained within the vial of "sVEGFR1/sFLT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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