Standard Curve (Sample)
Rat Fibronectin ELISA Kit | FN1 elisa kit
Rat Fibronectin ELISA Kit
Gene Names
Fn1; fn-1; FIBNEC
Reactivity
Rat
Applications
ELISA
Synonyms
Fibronectin; N/A; Rat Fibronectin ELISA Kit; FN; Cold-insoluble globulin; CIG; FN1 elisa kit
Reactivity
Rat
Specificity
The Rat Fibronectin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Fibronectin proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
2477
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
<15 pg/ml
Applicable Applications for FN1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FN1 elisa kit
Principle of the Assay: The Rat Fibronectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fibronectin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Fibronectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Fibronectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fibronectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fibronectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
259,350 Da
NCBI Official Full Name
Fibronectin
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
Fn1
NCBI Official Synonym Symbols
fn-1; FIBNEC
NCBI Protein Information
fibronectin; FN
UniProt Protein Name
Fibronectin
UniProt Gene Name
Fn1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_RAT
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Product Notes
The Rat FN1 fn1 (Catalog #AAA105814) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA105814 ELISA Kit recognizes Rat FN1. AAA Biotech's Fibronectin can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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