SDS-PAGE
Vitamin D Binding Protein (DBP) Recombinant Protein | DBP recombinant protein
Recombinant Vitamin D Binding Protein (DBP)
Gene Names
Gc; DBP; VDB
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Vitamin D Binding Protein (DBP); N/A; Recombinant Vitamin D Binding Protein (DBP); DBP recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-QMKHLSLLTT MSNRVCSQYA AYGKEKSRLS HLIKLAQKVP TANLENVLPL AEDFTEILSR CCESTSEDCM ASELPEHTIK ICQNLSKKNS KFEECCQENT PMNIFMCTYF MPAAEPLQLP AIKLPTGKDL CGQSTTQAMD QYTFELSRRT QVPEVFLSKV LEPTLKTLRE CCDTQDSVAC FSTQSPLLKR QLTSFIEKG
MGHHHHHHSGS-QMKHLSLLTT MSNRVCSQYA AYGKEKSRLS HLIKLAQKVP TANLENVLPL AEDFTEILSR CCESTSEDCM ASELPEHTIK ICQNLSKKNS KFEECCQENT PMNIFMCTYF MPAAEPLQLP AIKLPTGKDL CGQSTTQAMD QYTFELSRRT QVPEVFLSKV LEPTLKTLRE CCDTQDSVAC FSTQSPLLKR QLTSFIEKG
Sequence Length
476
Applicable Applications for DBP recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Gln205~Gly403 (Accession # P21614) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23.7kDa
NCBI Official Full Name
vitamin D-binding protein
NCBI Official Synonym Full Names
group specific component
NCBI Official Symbol
Gc
NCBI Official Synonym Symbols
DBP; VDB
NCBI Protein Information
vitamin D-binding protein; gc-globulin
UniProt Protein Name
Vitamin D-binding protein
UniProt Gene Name
Gc
UniProt Synonym Gene Names
DBP; VDB
UniProt Entry Name
VTDB_MOUSE
Similar Products
Product Notes
The DBP gc (Catalog #AAA134055) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vitamin D Binding Protein (DBP) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the DBP gc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG S-QMKHLSLL TT MSNRVCSQYA AYGKEKSRLS HLIKLAQKVP TANLENVLPL AEDFTEILSR CCESTSEDCM ASELPEHTIK ICQNLSKKNS KFEECCQENT PMNIFMCTYF MPAAEPLQLP AIKLPTGKDL CGQSTTQAMD QYTFELSRRT QVPEVFLSKV LEPTLKTLRE CCDTQDSVAC FSTQSPLLKR QLTSFIEKG. It is sometimes possible for the material contained within the vial of "Vitamin D Binding Protein (DBP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.