Glucokinase Regulator ELISA Kit | GCKR elisa kit
Glucokinase Regulator Colorimetric Cell-Based ELISA Kit
Gene Names
GCKR; GKRP; FGQTL5
Reactivity
Human, Mouse, Rat
Synonyms
Glucokinase Regulator; N/A; Glucokinase Regulator Colorimetric Cell-Based ELISA Kit; Glucokinase regulatory protein; Glucokinase regulator; GCKR; GCKR elisa kit
Reactivity
Human, Mouse, Rat
Specificity
The Anti-Glucokinase Regulator Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
Form/Format
One 96-Well Plate
Sequence Length
625
Preparation and Storage
Store at 4 degree C.
Related Product Information for GCKR elisa kit
Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Product Categories/Family for GCKR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68 kDa
NCBI Official Full Name
glucokinase regulatory protein
NCBI Official Synonym Full Names
glucokinase regulator
NCBI Official Symbol
GCKR
NCBI Official Synonym Symbols
GKRP; FGQTL5
NCBI Protein Information
glucokinase regulatory protein
UniProt Protein Name
Glucokinase regulatory protein
UniProt Gene Name
GCKR
UniProt Synonym Gene Names
GKRP; Glucokinase regulator
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Product Notes
The Human, Mouse, Rat GCKR gckr (Catalog #AAA315564) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA315564 ELISA Kit recognizes Human, Mouse, Rat GCKR. It is sometimes possible for the material contained within the vial of "Glucokinase Regulator, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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