Human growth differentiation factor 9 ELISA Kit | GDF9 elisa kit

Human growth differentiation factor-9, GDF-9 ELISA Kit

Cat. Num. AAA80667

• Reactivity: Human
• Synonyms: growth differentiation factor 9; N/A; Human growth differentiation factor-9, GDF-9 ELISA Kit; Human growth differentiation factor-9; GDF-9 ELISA Kit; OTTHUMP00000066213; GDF9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GDF9. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
• Sequence Length: 366
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 42.8 pg/ml-1500 pg/ml
• Sensitivity: 21.4 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for GDF9 elisa kit

Introduction: Growth differentiation factor-9 (GDF-9) is an oocyte-derived growth factor and a member of the transforming growth factor- (TGF-) superfamily that includes TGF-, activin, and bone morphogenetic proteins (BMPs). GDF-9 is indispensable for the development of ovarian follicles from the primary stage, and treatment with GDF-9 enhances the progression of early follicles into small preantral follicles. Similar to other TGF- family ligands, GDF-9 likely initiates signaling mediated by type I and type II receptors with serine/threonine kinase activity, followed by the phosphorylation of intracellular transcription factors named Smads.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GDF-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GDF-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GDF-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 572152931
• NCBI GeneID: 2661
• NCBI Accession #: NP_001275753.1
• NCBI GenBank Nucleotide #: NM_001288824.2
• UniProt Accession #: O60383; Q4VAW5
• Molecular Weight: 51,444 Da
• NCBI Official Full Name: growth/differentiation factor 9 isoform 2
• NCBI Official Synonym Full Names: growth differentiation factor 9
• NCBI Official Symbol: GDF9
• NCBI Protein Information: growth/differentiation factor 9; GDF-9
• UniProt Protein Name: Growth/differentiation factor 9
• UniProt Gene Name: GDF9
• UniProt Synonym Gene Names: GDF-9
• UniProt Entry Name: GDF9_HUMAN