General Uridine Triphosphate (UTP) ELISA Kit | UTP elisa kit
General Uridine Triphosphate (UTP) ELISA Kit
Reactivity
General
Synonyms
Uridine Triphosphate (UTP); N/A; General Uridine Triphosphate (UTP) ELISA Kit; UTP elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of UTP. No significant cross-reactivity or interference between UTP and analogues was observed.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
61.7-5,000ng/mL
Sensitivity
< 28.5ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UTP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UTP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for UTP elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to UTP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled UTP and unlabeled UTP (Standards or samples) with the pre-coated antibody specific to UTP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of UTP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of UTP in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of UTP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of UTP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
References
Epigenetic upregulation and functional role of the mitochondrial aspartate/glutamate carrier isoform 1 in hepatocellular carcinoma; Biochim Biophys Acta Mol Basis Dis; 2019 Jan;1865(1):38-47.; PMID: 30321589; Vittoria Infantino; Department of Science, University of Basilicata, viale dell"Ateneo Lucano 10, 85100 Potenza, Italy; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, via Orabona 4, 70125 Bari, Italy. Electronic address: vittoria.infantino@unibas.it.
