Human Growth Hormone ELISA Kit | GH1 elisa kit

Human Growth Hormone PicoKine ELISA Kit

Cat. Num. AAA45345

• Gene Names: GH1; GH; GHN; GH-N; hGH-N; IGHD1B
• Reactivity: Human
• Synonyms: Growth Hormone; N/A; Human Growth Hormone PicoKine ELISA Kit; Somatotropin; growth hormone 1; gH; GH-N; GH1; GHN; Growth hormone; Growth hormone 1; HGH; Pituitary growth hormone; SOMA_HUMAN; GH1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human GH
• Sequence Length: 217
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 2pg/ml

• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Growth Hormone PicoKine ELISA Kit standard curve)

Related Product Information for GH1 elisa kit

Principle of the assay: human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli,F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate.
Background: Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life.

NCBI and Uniprot Product Information

• NCBI GeneID: 2688
• UniProt Accession #: B1A4G6; P01241
• Molecular Weight: 24,847 Da
• NCBI Official Synonym Full Names: growth hormone 1
• NCBI Official Symbol: GH1
• NCBI Official Synonym Symbols: GH; GHN; GH-N; hGH-N; IGHD1B
• NCBI Protein Information: somatotropin; pituitary growth hormone
• UniProt Protein Name: Growth hormone 1 isoform 1
• UniProt Gene Name: GH1
• UniProt Entry Name: B1A4G6_HUMAN