Growth Hormone Recombinant Protein | GH1 recombinant protein

Mouse Growth Hormone Recombinant

Cat. Num. AAA76101

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Growth Hormone; N/A; Mouse Growth Hormone Recombinant; Somatotropin; gh1; gh-1; growth hormone; GH1 recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Mouse Growth Hormone was lyophilized from 0.2 um filtered 100mM NaCl, 20mM PB, pH 7.4.
• Sequence: MATDSRTSWL LTVSLLCLLW PQEASAFPAM PLSSLFSNAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKE EAQQRTDMEL LRFSLLLIQS WLGPVQFLSR IFTNSLMFGT SDRVYEKLKD LEEGIQALMQ ELEDGSPRVG QILKQTYDKF DANMRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFV ESSCAF
• Sequence Length: 216

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Mouse Growth Hormone mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native Mouse Growth Hormone is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa.; ; Recombinant GH is a disulfide-linked homodimeric protein consisting of 191 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for GH1 recombinant protein

The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the Cytokine receptor families. Growth hormone has been shown to be produced by T- cells, B-cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T- helper cells.

Product Categories/Family for GH1 recombinant protein

Cytokines

References

• Growth Hormone secretagogues and Growth Hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the g protein go1 by the ghrelin receptor .Mol. Pharmacol., Oct 2009; 76: 802 - 811.
• statement by the Growth Hormone research society on the gh/igf-i axis in extending health span .J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044.
• the p561t polymorphism of the Growth Hormone receptor gene has an inhibitory effect on mandibular growth in young children .Eur J Orthod, Oct 2009; 31: 536 - 541.
• central ghrelin regulates peripheral lipid metabolism in a Growth Hormone-independent fashion .Endocrinology, Oct 2009; 150: 4562 - 4574.
• effects of syndyphalin-33 on feed intake and circulating measures of Growth Hormone, cortisol, and immune cell populations in the recently weaned pig .J Anim Sci, Oct 2009; 87: 3218 - 3225.