Goat Arrestin beta 2 Antibody | anti-ARRB2 antibody

Goat anti-Arrestin beta 2 Antibody

Cat. Num. AAA13649

• Gene Names: ARRB2; ARB2; ARR2; BARR2
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Arrestin beta 2; N/A; Goat anti-Arrestin beta 2 Antibody; arrestin, beta 2, ARRB2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3, arrestin beta-2; anti-ARRB2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

• Applicable Applications for anti-ARRB2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.; Western blot: Approx 45kDa band observed in lysates of cell lines A549 and U251 and in preliminary testing of KNRK cell lysate (calculated MW of 46.1kDa according to NP_004304.1 and 44.4kDa according to NP_945355.1) Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.; Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10µg/ml.; Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Important Comments: This antibody is expected to recognize both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
• Immunogen: Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1.; NP_001316993.1; Please note the peptide is available for sale.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

FCM (Flow Cytometry)

(AAA13649 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence)

(AAA13649 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IHC (Immunohistochemistry)

((4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4757780
• NCBI GeneID: 409
• NCBI Accession #: NP_004304.1; NP_945355.1
• NCBI GenBank Nucleotide #: NM_004313.3
• NCBI Official Full Name: beta-arrestin-2 isoform 1
• NCBI Official Synonym Full Names: arrestin beta 2
• NCBI Official Symbol: ARRB2
• NCBI Official Synonym Symbols: ARB2; ARR2; BARR2
• NCBI Protein Information: beta-arrestin-2
• UniProt Protein Name: Beta-arrestin-2
• UniProt Gene Name: ARRB2
• UniProt Synonym Gene Names: ARB2; ARR2
• UniProt Entry Name: ARRB2_HUMAN