WB (Western Blot)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MIB1 with C-terminal MYC tag probed with AAA61872 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AAA61872 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat Mindbomb 1/MIB1 Antibody | anti-MIB1 antibody
Goat anti-Mindbomb 1/MIB1 Antibody
Gene Names
MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
Reactivity
HumanExpected from sequence similarity: Mouse, Rat, Dog, Cow
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Mindbomb 1/MIB1; N/A; Goat anti-Mindbomb 1/MIB1 Antibody; MIB1; mindbomb homolog 1 (Drosophila); DIP-1; DKFZp686I0769; DKFZp761M1710; FLJ90676; MGC129659; MGC129660; MIB; ZZANK2; ZZZ6; DAPK-interacting protein 1; E3 ubiquitin-protein ligase MIB1; OTTHUMP00000162549; mind bomb homolog 1; ubiquitin ligase mind bom; anti-MIB1 antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Mouse, Rat, Dog, Cow
Expected from sequence similarity: Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Applicable Applications for anti-MIB1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-DKDNTNVNADVQKLQ, from the internal region (near C Terminus) of the protein sequence according to NP_065825.1.
Epitope
internal region (near C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MIB1 with C-terminal MYC tag probed with AAA61872 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AAA61872 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MIB1 with C-terminal MYC tag probed with AAA61872 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AAA61872 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
IHC (Immunohistochemistry)
(AAA61872 (2ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
(AAA61872 (2ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
References
Berndt JD, Aoyagi A, Yang P, Anastas JN, Tang L, Moon RT.; Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/beta-catenin signaling.; J Cell Biol. 2011 Sep 5;194(5):737-50.; PMID: 21875946
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110,136 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MIB1
NCBI Official Synonym Full Names
mindbomb E3 ubiquitin protein ligase 1
NCBI Official Symbol
MIB1
NCBI Official Synonym Symbols
MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
NCBI Protein Information
E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2
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Product Notes
The MIB1 (Catalog #AAA61872) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Mindbomb 1/MIB1 Antibody reacts with Human Expected from sequence similarity: Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Mindbomb 1/MIB1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the MIB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mindbomb 1/MIB1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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