IF (Immunofluorescence)
(Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.)
Rabbit MIB1 Polyclonal Antibody | anti-MIB1 antibody
MIB1 Antibody
Gene Names
MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
MIB1 antibody is affinity chromatography purified via peptide column.
Synonyms
MIB1, Antibody; MIB1 Antibody; MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2; KIAA1323; E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; mindbomb E3 ubiquitin protein ligase 1; anti-MIB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.
Purity/Purification
MIB1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1006
Applicable Applications for anti-MIB1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1.
Buffer
MIB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
MIB1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 μg/ml.)
Related Product Information for anti-MIB1 antibody
The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 89; 102; 111 kDa
Observed: 103 kDa
Observed: 103 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase MIB1
NCBI Official Synonym Full Names
mindbomb E3 ubiquitin protein ligase 1
NCBI Official Symbol
MIB1
NCBI Official Synonym Symbols
MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
NCBI Protein Information
E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2
UniProt Protein Name
E3 ubiquitin-protein ligase MIB1
UniProt Gene Name
MIB1
UniProt Synonym Gene Names
DIP1; KIAA1323; ZZANK2; DIP-1
UniProt Entry Name
MIB1_HUMAN
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Product Notes
The MIB1 mib1 (Catalog #AAA40480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIB1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MIB1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MIB1 mib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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