FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)
Goat PDGFB Precursor Antibody | anti-PDGFB antibody
Goat Anti-PDGFB Precursor Antibody
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Reactivity
HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PDGFB Precursor; N/A; Goat Anti-PDGFB Precursor Antibody; PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); becaplermin; oncogene SIS; platelet-derived growth factor 2; platelet-derived growth factor beta; platelet-derived growth factor, B chain; v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog); anti-PDGFB antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
241
Applicable Applications for anti-PDGFB antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-KHRKFKHTHDKT, from the C Terminus of the protein sequence according to NP_002599.1; NP_148937.1.
Peptide Sequence
KHRKFKHTHDKT
Epitope
C Terminus
Important comments
This antibody is expected to recognize isoform 1 and 2 (NP_002599.1; NP_148937.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue))
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue))
References
Tchougounova E, Jiang Y, Bråsäter D, Lindberg N, Kastemar M, Asplund A, Westermark B, Uhrbom L.: Sox5 can suppress platelet-derived growth factor B-induced glioma development in Ink4a-deficient mice through induction of acute cellular senescence. Oncogene 2009 Mar 28 (12): 1537-48. PMID: 19219070
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet-derived growth factor subunit B isoform 1 preproprotein
NCBI Official Synonym Full Names
platelet derived growth factor subunit B
NCBI Official Symbol
PDGFB
NCBI Official Synonym Symbols
SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
NCBI Protein Information
platelet-derived growth factor subunit B
UniProt Protein Name
Platelet-derived growth factor subunit B
UniProt Gene Name
PDGFB
UniProt Synonym Gene Names
PDGF2; SIS; PDGF subunit B
UniProt Entry Name
PDGFB_HUMAN
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Product Notes
The PDGFB pdgfb (Catalog #AAA61834) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-PDGFB Precursor Antibody reacts with Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFB Precursor can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the PDGFB pdgfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFB Precursor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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