Goat SHH Antibody | anti-SHH antibody

Goat Anti-SHH Antibody

Cat. Num. AAA61823

• Gene Names: SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
• Reactivity: Human, Mouse; Expected from sequence similarity: Human, Rat, Dog
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SHH; N/A; Goat Anti-SHH Antibody; sonic hedgehog homolog (Drosophila); HGNC:10848; HHG1; HLP3; HPE3; SMMCI ; sonic hedgehog; anti-SHH antibody

• Host: Goat
• Reactivity: Human, Mouse; Expected from sequence similarity: Human, Rat, Dog
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 462

• Applicable Applications for anti-SHH antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence C-DSEALHPLGMAVK, from the C Terminus of the protein sequence according to NP_000184.1.
• Peptide Sequence: DSEALHPLGMAVK
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (bl)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue)

WB (Western Blot)

((2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

References

Ahn S, Joyner AL.: In vivo analysis of quiescent adult neural stem cells responding to Sonic hedgehog. Nature. 2005 Oct 6;437(7060):894-7. PMID: 16208373

NCBI and Uniprot Product Information

• NCBI GI #: 4506939
• NCBI GeneID: 6469
• NCBI Accession #: NP_000184.1
• NCBI GenBank Nucleotide #: NP_000184.1
• UniProt Accession #: Q15465
• NCBI Official Full Name: sonic hedgehog protein isoform 1 preproprotein
• NCBI Official Synonym Full Names: sonic hedgehog signaling molecule
• NCBI Official Symbol: SHH
• NCBI Official Synonym Symbols: TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
• NCBI Protein Information: sonic hedgehog protein
• UniProt Protein Name: Sonic hedgehog protein
• UniProt Gene Name: SHH
• UniProt Synonym Gene Names: SHH
• UniProt Entry Name: SHH_HUMAN