Goat GATA1 Antibody | anti-GATA1 antibody

Goat Anti-GATA1 Antibody

Cat. Num. AAA61819

• Gene Names: GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• Reactivity: Human; Expected from sequence similarity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GATA1; N/A; Goat Anti-GATA1 Antibody; GATA binding protein 1 (globin transcription factor 1); GF1; NFE1; ERYF1; anti-GATA1 antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Sequence Length: 413

• Applicable Applications for anti-GATA1 antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence C-PTTSTTVVAPLSS, from the C Terminus of the protein sequence according to NP_002040.1.
• Peptide Sequence: C-PTTSTTVVAPLSS
• Negative Control: Human Hippocampus lysate.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

WB (Western Blot)

((2ug/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503925
• NCBI GeneID: 2623
• NCBI Accession #: NP_002040.1
• NCBI GenBank Nucleotide #: NP_002040.1
• UniProt Accession #: P15976
• NCBI Official Full Name: erythroid transcription factor
• NCBI Official Synonym Full Names: GATA binding protein 1
• NCBI Official Symbol: GATA1
• NCBI Official Synonym Symbols: GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• NCBI Protein Information: erythroid transcription factor
• UniProt Protein Name: Erythroid transcription factor
• UniProt Gene Name: GATA1
• UniProt Synonym Gene Names: ERYF1; GF1; GATA-1; GF-1
• UniProt Entry Name: GATA1_HUMAN