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product-image-AAA60869_IHC10.jpg IHC (Immunohistochemistry) (Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody)

Goat FOXP3/Scurfin Antibody | anti-Foxp3 antibody

Goat anti-FOXP3/Scurfin (Mouse) Antibody

Gene Names
Foxp3; sf; JM2; scurfin
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FOXP3/Scurfin; N/A; Goat anti-FOXP3/Scurfin (Mouse) Antibody; Foxp3; forkhead box P3; sf; JM2; scurfin; scurfy; RP23-54C14.1; OTTMUSP00000017952; OTTMUSP00000017961; OTTMUSP00000017962; Foxp3 antibody; forkhead box P3 antibody; sf antibody; JM2 antibody; scurfin antibody; scurfy antibody; RP23-54C14.1 antibody; OTTMUSP00000017952 antibody; OTTMUSP00000017961 antibody; OTTMUSP00000017962 antibody; Scurfin; FOXP3 / Scurfin (Mouse); anti-Foxp3 antibody
Ordering
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
429
Applicable Applications for anti-Foxp3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.

IHC (Immunohistochemistry)

(Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody)

product-image-AAA60869_IHC10.jpg IHC (Immunohistochemistry) (Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody)

IHC (Immunohistochemisry)

((5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

product-image-AAA60869_IHC11.jpg IHC (Immunohistochemisry) ((5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

FCM/FACS (Flow Cytometry)

(AAA60869 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

product-image-AAA60869_FCM13.jpg FCM/FACS (Flow Cytometry) (AAA60869 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

product-image-AAA60869_IF15.jpg IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
forkhead box protein P3
NCBI Official Synonym Full Names
forkhead box P3
NCBI Official Symbol
Foxp3
NCBI Official Synonym Symbols
sf; JM2; scurfin
NCBI Protein Information
forkhead box protein P3
UniProt Protein Name
Forkhead box protein P3
UniProt Gene Name
Foxp3
UniProt Entry Name
FOXP3_MOUSE

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Product Notes

The Foxp3 foxp3 (Catalog #AAA60869) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXP3/Scurfin (Mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FOXP3/Scurfin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the Foxp3 foxp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXP3/Scurfin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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