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IF (Immunofluorescence)
(Staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).)
Goat FOXP3/SCURFIN Polyclonal Antibody | anti-FOXP3 antibody
Goat anti-FOXP3/SCURFIN Antibody
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FOXP3/SCURFIN, Antibody; Goat anti-FOXP3/SCURFIN Antibody; FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin; FOXP3 antibody; SCURFIN antibody; forkhead box P3 antibody; JM2 antibody; AIID antibody; IPEX antibody; PIDX antibody; XPID antibody; DIETER antibody; JM2 protein antibody; immunodeficiency; polyendocrinopathy; enteropathy; X-linked antibody; immune dysregulation; MGC141961 antibody; MGC141963 antibody; FOXP3delta7 antibody; scurfin antibody; FOXP3 / SCURFIN; anti-FOXP3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SQRPSRCSNPTPGP
Sequence Length
431
Applicable Applications for anti-FOXP3 antibody
ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IF (Immunofluorescence)
(Staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).)
IF (Immunofluorescence)
(Staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
WB (Western Blot)
((1ug/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1ug/ml) negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
((1ug/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1ug/ml) negative control Pancreas (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
forkhead box protein P3 isoform a
NCBI Official Synonym Full Names
forkhead box P3
NCBI Official Symbol
FOXP3
NCBI Official Synonym Symbols
JM2; AIID; IPEX; PIDX; XPID; DIETER
NCBI Protein Information
forkhead box protein P3
UniProt Protein Name
Forkhead box protein P3
UniProt Gene Name
FOXP3
UniProt Synonym Gene Names
IPEX
UniProt Entry Name
FOXP3_HUMAN
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Product Notes
The FOXP3 foxp3 (Catalog #AAA61014) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXP3/SCURFIN Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXP3/SCURFIN can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the FOXP3 foxp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQRPSRCSNP TPGP. It is sometimes possible for the material contained within the vial of "FOXP3/SCURFIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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