FCM/FACS (Flow Cytometry)
(Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/mL, blue line) or CD8A Rabbit mAb (A0663, 10 ug/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AAA283339, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).)
F (ab')? Fragment anti-IgG, Fc fragment specific Secondary Antibody
FITC-conjugated F (ab')? Fragment Goat anti-Rabbit IgG, Fc fragment specific
Reactivity
Rabbit
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
F (ab')? Fragment anti-IgG, Fc fragment specific; N/A; FITC-conjugated F (ab')? Fragment Goat anti-Rabbit IgG, Fc fragment specific; F (ab')? Fragment anti-IgG, Fc fragment specific secondary antibody
Host
Goat
Reactivity
Rabbit
Isotype
Fluorescein conjugated IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Applicable Applications for F (ab')? Fragment anti-IgG, Fc fragment specific secondary antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Rabbit IgG
Conjugation
FITC. Ex:491nm. Em:516nm.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/mL, blue line) or CD8A Rabbit mAb (A0663, 10 ug/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AAA283339, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/mL, blue line) or CD8A Rabbit mAb (A0663, 10 ug/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AAA283339, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).)
ICC (Immunocytochemistry)
(Confocal imaging of SH-SY5Y cells using ?III-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')? Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AAA283339, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of SH-SY5Y cells using ?III-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')? Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AAA283339, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of paraffin-embedded Mouse brain using ?III-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')? Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AAA283339, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)
ICC (Immunocytochemistry)
(Confocal imaging of paraffin-embedded Mouse brain using ?III-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')? Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AAA283339, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)
Related Product Information for F (ab')? Fragment anti-IgG, Fc fragment specific secondary antibody
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies. Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Product Categories/Family for F (ab')? Fragment anti-IgG, Fc fragment specific secondary antibody
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Product Notes
The F (ab')? Fragment anti-IgG, Fc fragment specific (Catalog #AAA283339) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The FITC-conjugated F (ab')? Fragment Goat anti-Rabbit IgG, Fc fragment specific reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's F (ab')? Fragment anti-IgG, Fc fragment specific can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the F (ab')? Fragment anti-IgG, Fc fragment specific for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F (ab')? Fragment anti-IgG, Fc fragment specific, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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