ELISA
(Biotinylated Human GPA33/A33 Protein ELISA DataImmobilized Anti-GPA33 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA.)
GPC3/Glypican 3 Recombinant Protein | GPC3 recombinant protein
Biotinylated Human GPC3/Glypican 3 Protein Recombinant
Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
GPC3/Glypican 3; N/A; Biotinylated Human GPC3/Glypican 3 Protein Recombinant; GPC3 recombinant protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human GPC3/Glypican 3 was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human GPC3/Glypican 3 including a C-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Protease-inhibitor
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
ELISA
(Biotinylated Human GPA33/A33 Protein ELISA DataImmobilized Anti-GPA33 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA.)
ELISA
(Biotinylated Human GPA33/A33 Protein ELISA DataImmobilized Anti-GPA33 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA.)
Application Data
(Biotinylated Human GPA33/A33 Protein SEC-HPLCThe purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.)
Application Data
(Biotinylated Human GPA33/A33 Protein SEC-HPLCThe purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE
(Biotinylated Human GPA33/A33 Protein Tris-Bis PAGEBiotinylated Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated Human GPA33/A33 Protein Tris-Bis PAGEBiotinylated Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Product Categories/Family for GPC3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,563 Da
NCBI Official Full Name
glypican-3 isoform 4
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
GPC3
NCBI Official Synonym Symbols
SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
NCBI Protein Information
glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan
UniProt Protein Name
Glypican-3
UniProt Gene Name
GPC3
UniProt Synonym Gene Names
OCI5
UniProt Entry Name
GPC3_HUMAN
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Product Notes
The GPC3 gpc3 (Catalog #AAA76141) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GPC3/Glypican 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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