Bovine Platelet Membrane Glycoprotein 2b3a/CD41+CD61 ELISA Kit | GPIIbIIIa/CD41+CD61 elisa kit

Bovine Platelet Membrane Glycoprotein 2b3a/CD41+CD61 ELISA Kit

Cat. Num. AAA16900

• Reactivity: Bovine
• Synonyms: Platelet Membrane Glycoprotein 2b3a/CD41+CD61; N/A; Bovine Platelet Membrane Glycoprotein 2b3a/CD41+CD61 ELISA Kit; GPIIbIIIa/CD41+CD61 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of GP2b3a. No significant cross-reactivity or interference between GP2b3a and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GP2b3a and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GPIIbIIIa/CD41+CD61 elisa kit

Principle of the Assay: GP2b3a ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GP2b3a antibody and an GP2b3a-HRP conjugate. The assay sample and buffer are incubated together with GP2b3a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GP2b3a concentration since GP2b3a from samples and GP2b3a-HRP conjugate compete for the anti-GP2b3a antibody binding site. Since the number of sites is limited, as more sites are occupied by GP2b3a from the sample, fewer sites are left to bind GP2b3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP2b3a concentration in each sample is interpolated from this standard curve.

Intended Uses: This GP2b3a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GP2b3a. This ELISA kit for research use only, not for therapeutic or diagnostic applications!