Standard Curve (Sample)
Mouse Gremlin 1 ELISA Kit | GREM1 elisa kit
Mouse Gremlin 1 ELISA Kit
Gene Names
Grem1; ld; Drm; Grem; Cktsf1b1
Reactivity
Mouse
Synonyms
Gremlin 1; N/A; Mouse Gremlin 1 ELISA Kit; CKTSF1B1; DAND2; DRM; Gremlin-1; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1 BMP antagonist 1; DAN domain family member 2; Down-regulated in Mos-transformed cells protein; Increased in high glucose protein 2; IHG-2; GREM1 elisa kit
Reactivity
Mouse
Specificity
The Mouse Gremlin 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Gremlin 1 proteins within the range of 93.8 pg/ml - 6000 pg/ml.
Sequence Length
184
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GREM1 elisa kit
Principle of the Assay: The Mouse Gremlin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Gremlin 1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Gremlin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gremlin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Gremlin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gremlin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Gremlin, also known as Increased in High Glucose protein 2 (IHG-2) and Down-regulated in Mos-transformed cells protein (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Human Gremlin is synthesized as a 184 amino acid (aa) precursor that contains a 24 aa signal sequence and a 160 aa mature region. The mature region contains one potential site for N-linked glycosylation (Asn 42), a cysteine-rich region, and a cysteine knot motif (aa 94 - 184) whose structure is shared by members of the TGF-beta superfamily. Post-translational modifications include glycosylation and phosphorylation. Gremlin exists in both secreted and membrane-associated forms. There are two isoforms for human Gremlin. Isoform 1 is the standard protein, and in isoform 2, there is a deletion of aa 39 - 79. Human Gremlin shares 99% and 86% aa sequence identity with mouse and chick Gremlin, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle. Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP-2, BMP-4, and BMP-7, thus preventing them from interacting with their cell surface receptors. This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development and to play a role in human diseases, such as diabetic nephropathy. However, intracellular BMP-independent mechanisms of action may mediate the ability of Gremlin to suppress transformation and tumorigenesis under certain experimental conditions. Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis. In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium.
Background: Gremlin, also known as Increased in High Glucose protein 2 (IHG-2) and Down-regulated in Mos-transformed cells protein (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Human Gremlin is synthesized as a 184 amino acid (aa) precursor that contains a 24 aa signal sequence and a 160 aa mature region. The mature region contains one potential site for N-linked glycosylation (Asn 42), a cysteine-rich region, and a cysteine knot motif (aa 94 - 184) whose structure is shared by members of the TGF-beta superfamily. Post-translational modifications include glycosylation and phosphorylation. Gremlin exists in both secreted and membrane-associated forms. There are two isoforms for human Gremlin. Isoform 1 is the standard protein, and in isoform 2, there is a deletion of aa 39 - 79. Human Gremlin shares 99% and 86% aa sequence identity with mouse and chick Gremlin, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle. Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP-2, BMP-4, and BMP-7, thus preventing them from interacting with their cell surface receptors. This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development and to play a role in human diseases, such as diabetic nephropathy. However, intracellular BMP-independent mechanisms of action may mediate the ability of Gremlin to suppress transformation and tumorigenesis under certain experimental conditions. Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis. In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,710 Da
NCBI Official Full Name
gremlin-1
NCBI Official Synonym Full Names
gremlin 1, DAN family BMP antagonist
NCBI Official Symbol
Grem1
NCBI Official Synonym Symbols
ld; Drm; Grem; Cktsf1b1
NCBI Protein Information
gremlin-1
UniProt Protein Name
Gremlin-1
UniProt Gene Name
Grem1
UniProt Synonym Gene Names
Cktsf1b1; Drm
UniProt Entry Name
GREM1_MOUSE
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Product Notes
The Mouse GREM1 grem1 (Catalog #AAA263122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263122 ELISA Kit recognizes Mouse GREM1. It is sometimes possible for the material contained within the vial of "Gremlin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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