Mouse Progranulin ELISA Kit | GRN elisa kit

Mouse Progranulin PicoKine Fast ELISA Kit

Cat. Num. AAA125357

• Gene Names: Grn; GP88; PEPI; Pgrn; PCDGF; epithelin
• Reactivity: Mouse
• Synonyms: Progranulin; N/A; Mouse Progranulin PicoKine Fast ELISA Kit; mouse Progranulin; Granulins; PC cell-derived growth factor; PCDGF; Proepithelin; PEPI; Acrogranin; Granulin-1; Granulin-2; Granulin-3; Granulin-4; Granulin-5; Granulin-6; Granulin-7; Grn; GRN elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Grn
• Sequence Length: 589
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 0.78 ng/ml - 50 ng/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for GRN elisa kit

Principle of the Assay: The Picokine™ Mouse Grn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Grn with a 96-well strip plate that is pre-coated with antibody specific for Grn. The detection antibody is a biotinylated antibody specific for Grn. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Grn with immunogen: Expression system for standard: NS0; Immunogen sequence: T18-L589. The kit is analytically validated with ready to use reagents. To measure Mouse Grn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Grn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Grn in the sample.

Background/Introduction: Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 585216
• NCBI GeneID: 14824
• UniProt Accession #: P28798
• NCBI Official Full Name: Progranulin
• NCBI Official Synonym Full Names: granulin
• NCBI Official Symbol: Grn
• NCBI Official Synonym Symbols: GP88; PEPI; Pgrn; PCDGF; epithelin
• NCBI Protein Information: progranulin
• UniProt Protein Name: Granulins
• UniProt Gene Name: Grn
• UniProt Synonym Gene Names: PCDGF; PEPI
• UniProt Entry Name: GRN_MOUSE