Standard Curve (Sample)
Rat Glycogen Synthase Kinase 3 Beta ELISA Kit | GSK-3beta elisa kit
Rat Glycogen Synthase Kinase 3 Beta ELISA Kit
Reactivity
Rat
Synonyms
Glycogen Synthase Kinase 3 Beta; N/A; Rat Glycogen Synthase Kinase 3 Beta ELISA Kit; GSK-3beta elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GSK3beta. No significant cross-reactivity or interference between GSK3beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSK3beta and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GSK-3beta elisa kit
Intended Uses: This GSK3beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GSK3beta. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: GSK3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSK3beta antibody and an GSK3beta-HRP conjugate. The assay sample and buffer are incubated together with GSK3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSK3beta concentration since GSK3beta from samples and GSK3beta-HRP conjugate compete for the anti-GSK3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by GSK3beta from the sample, fewer sites are left to bind GSK3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3beta concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GSK3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSK3beta antibody and an GSK3beta-HRP conjugate. The assay sample and buffer are incubated together with GSK3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSK3beta concentration since GSK3beta from samples and GSK3beta-HRP conjugate compete for the anti-GSK3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by GSK3beta from the sample, fewer sites are left to bind GSK3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3beta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,034 Da
NCBI Official Full Name
Glycogen synthase kinase-3 beta
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
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Product Notes
The Rat GSK-3beta gsk3b (Catalog #AAA246995) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246995 ELISA Kit recognizes Rat GSK-3beta. It is sometimes possible for the material contained within the vial of "Glycogen Synthase Kinase 3 Beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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